Rat ELISA Kits Search Results


rat's specific elisa kits cat. no. mbs2268427  (MyBiosource Biotechnology)
90
MyBiosource Biotechnology rat's specific elisa kits cat. no. mbs2268427
Rat's Specific Elisa Kits Cat. No. Mbs2268427, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+ELISA+Kits/10__1016_slash_j__jff__2022__105058-86-27-44?v=MyBiosource+Biotechnology
Average 90 stars, based on 1 article reviews
rat's specific elisa kits cat. no. mbs2268427 - by Bioz Stars, 2026-07
90/100 stars
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human and rat pten elisa kits  (ELK Biotechnology)
90
ELK Biotechnology human and rat pten elisa kits
Differential expressions of <t>PTEN</t> in the human and rat corneal endothelium. ( a ) Immunofluorescence staining of PTEN ( red ) and actin detected by phalloidin antibody ( green ) in human and rat corneas. Cell nuclei were stained with DAPI ( blue ). Scale bar = 20 µm. N = 3. ( b ) Flat-mount staining of PTEN ( red ) in human and rat corneal endothelium. Cell nuclei were stained with DAPI ( blue ). Scale bar = 10 µm. N = 3. ( c, d ) Total protein was extracted from the corneal endothelium detached from human corneoscleral rims and rat corneas. Cell lysates were analyzed by western blotting and immunoblotted using an anti-PTEN antibody. Data are presented as the mean ± SD from three independent experiments and plotted as PTEN protein expression compared with the respective GAPDH control. ** P < 0.01 by the Student's t -test. ( e ) Total protein extracted from human ( N = 9) and rat ( N = 6) corneal endothelium tissue were analyzed by <t>ELISA.</t> Data are presented as the mean ± SD from three independent experiments and plotted as concentration of PTEN in the corneal endothelium (ng/mL). *** P < 0.001 by the Student's t -test.
Human And Rat Pten Elisa Kits, supplied by ELK Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+ELISA+Kits/pmc07425707-32-16-20?v=ELK+Biotechnology
Average 90 stars, based on 1 article reviews
human and rat pten elisa kits - by Bioz Stars, 2026-07
90/100 stars
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rat elisa kits  (BioDiagnostics Inc)
90
BioDiagnostics Inc rat elisa kits
Differential expressions of <t>PTEN</t> in the human and rat corneal endothelium. ( a ) Immunofluorescence staining of PTEN ( red ) and actin detected by phalloidin antibody ( green ) in human and rat corneas. Cell nuclei were stained with DAPI ( blue ). Scale bar = 20 µm. N = 3. ( b ) Flat-mount staining of PTEN ( red ) in human and rat corneal endothelium. Cell nuclei were stained with DAPI ( blue ). Scale bar = 10 µm. N = 3. ( c, d ) Total protein was extracted from the corneal endothelium detached from human corneoscleral rims and rat corneas. Cell lysates were analyzed by western blotting and immunoblotted using an anti-PTEN antibody. Data are presented as the mean ± SD from three independent experiments and plotted as PTEN protein expression compared with the respective GAPDH control. ** P < 0.01 by the Student's t -test. ( e ) Total protein extracted from human ( N = 9) and rat ( N = 6) corneal endothelium tissue were analyzed by <t>ELISA.</t> Data are presented as the mean ± SD from three independent experiments and plotted as concentration of PTEN in the corneal endothelium (ng/mL). *** P < 0.001 by the Student's t -test.
Rat Elisa Kits, supplied by BioDiagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+ELISA+Kits/pmc11752902-60-1-5?v=BioDiagnostics+Inc
Average 90 stars, based on 1 article reviews
rat elisa kits - by Bioz Stars, 2026-07
90/100 stars
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commercial rat specific elisa kits  (Cloud-Clone corp)
90
Cloud-Clone corp commercial rat specific elisa kits
Differential expressions of <t>PTEN</t> in the human and rat corneal endothelium. ( a ) Immunofluorescence staining of PTEN ( red ) and actin detected by phalloidin antibody ( green ) in human and rat corneas. Cell nuclei were stained with DAPI ( blue ). Scale bar = 20 µm. N = 3. ( b ) Flat-mount staining of PTEN ( red ) in human and rat corneal endothelium. Cell nuclei were stained with DAPI ( blue ). Scale bar = 10 µm. N = 3. ( c, d ) Total protein was extracted from the corneal endothelium detached from human corneoscleral rims and rat corneas. Cell lysates were analyzed by western blotting and immunoblotted using an anti-PTEN antibody. Data are presented as the mean ± SD from three independent experiments and plotted as PTEN protein expression compared with the respective GAPDH control. ** P < 0.01 by the Student's t -test. ( e ) Total protein extracted from human ( N = 9) and rat ( N = 6) corneal endothelium tissue were analyzed by <t>ELISA.</t> Data are presented as the mean ± SD from three independent experiments and plotted as concentration of PTEN in the corneal endothelium (ng/mL). *** P < 0.001 by the Student's t -test.
Commercial Rat Specific Elisa Kits, supplied by Cloud-Clone corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+ELISA+Kits/pmc07758008-69-18-22?v=Cloud-Clone+corp
Average 90 stars, based on 1 article reviews
commercial rat specific elisa kits - by Bioz Stars, 2026-07
90/100 stars
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rat lh elisa kits  (Abnova)
90
Abnova rat lh elisa kits
Differential expressions of <t>PTEN</t> in the human and rat corneal endothelium. ( a ) Immunofluorescence staining of PTEN ( red ) and actin detected by phalloidin antibody ( green ) in human and rat corneas. Cell nuclei were stained with DAPI ( blue ). Scale bar = 20 µm. N = 3. ( b ) Flat-mount staining of PTEN ( red ) in human and rat corneal endothelium. Cell nuclei were stained with DAPI ( blue ). Scale bar = 10 µm. N = 3. ( c, d ) Total protein was extracted from the corneal endothelium detached from human corneoscleral rims and rat corneas. Cell lysates were analyzed by western blotting and immunoblotted using an anti-PTEN antibody. Data are presented as the mean ± SD from three independent experiments and plotted as PTEN protein expression compared with the respective GAPDH control. ** P < 0.01 by the Student's t -test. ( e ) Total protein extracted from human ( N = 9) and rat ( N = 6) corneal endothelium tissue were analyzed by <t>ELISA.</t> Data are presented as the mean ± SD from three independent experiments and plotted as concentration of PTEN in the corneal endothelium (ng/mL). *** P < 0.001 by the Student's t -test.
Rat Lh Elisa Kits, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+ELISA+Kits/pmc07443349-93-27-31?v=Abnova
Average 90 stars, based on 1 article reviews
rat lh elisa kits - by Bioz Stars, 2026-07
90/100 stars
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rat elisa kit  (MyBiosource Biotechnology)
90
MyBiosource Biotechnology rat elisa kit
Differential expressions of <t>PTEN</t> in the human and rat corneal endothelium. ( a ) Immunofluorescence staining of PTEN ( red ) and actin detected by phalloidin antibody ( green ) in human and rat corneas. Cell nuclei were stained with DAPI ( blue ). Scale bar = 20 µm. N = 3. ( b ) Flat-mount staining of PTEN ( red ) in human and rat corneal endothelium. Cell nuclei were stained with DAPI ( blue ). Scale bar = 10 µm. N = 3. ( c, d ) Total protein was extracted from the corneal endothelium detached from human corneoscleral rims and rat corneas. Cell lysates were analyzed by western blotting and immunoblotted using an anti-PTEN antibody. Data are presented as the mean ± SD from three independent experiments and plotted as PTEN protein expression compared with the respective GAPDH control. ** P < 0.01 by the Student's t -test. ( e ) Total protein extracted from human ( N = 9) and rat ( N = 6) corneal endothelium tissue were analyzed by <t>ELISA.</t> Data are presented as the mean ± SD from three independent experiments and plotted as concentration of PTEN in the corneal endothelium (ng/mL). *** P < 0.001 by the Student's t -test.
Rat Elisa Kit, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+ELISA+Kits/pm37450014-56-8-12?v=MyBiosource+Biotechnology
Average 90 stars, based on 1 article reviews
rat elisa kit - by Bioz Stars, 2026-07
90/100 stars
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rebisu insulin rat elisa kits  (Shibayagi ltd)
90
Shibayagi ltd rebisu insulin rat elisa kits
Differential expressions of <t>PTEN</t> in the human and rat corneal endothelium. ( a ) Immunofluorescence staining of PTEN ( red ) and actin detected by phalloidin antibody ( green ) in human and rat corneas. Cell nuclei were stained with DAPI ( blue ). Scale bar = 20 µm. N = 3. ( b ) Flat-mount staining of PTEN ( red ) in human and rat corneal endothelium. Cell nuclei were stained with DAPI ( blue ). Scale bar = 10 µm. N = 3. ( c, d ) Total protein was extracted from the corneal endothelium detached from human corneoscleral rims and rat corneas. Cell lysates were analyzed by western blotting and immunoblotted using an anti-PTEN antibody. Data are presented as the mean ± SD from three independent experiments and plotted as PTEN protein expression compared with the respective GAPDH control. ** P < 0.01 by the Student's t -test. ( e ) Total protein extracted from human ( N = 9) and rat ( N = 6) corneal endothelium tissue were analyzed by <t>ELISA.</t> Data are presented as the mean ± SD from three independent experiments and plotted as concentration of PTEN in the corneal endothelium (ng/mL). *** P < 0.001 by the Student's t -test.
Rebisu Insulin Rat Elisa Kits, supplied by Shibayagi ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+ELISA+Kits/pm21150098-33-22-27?v=Shibayagi+ltd
Average 90 stars, based on 1 article reviews
rebisu insulin rat elisa kits - by Bioz Stars, 2026-07
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sandwich-type elisa kit for rats  (USCN Life)
90
USCN Life sandwich-type elisa kit for rats
Differential expressions of <t>PTEN</t> in the human and rat corneal endothelium. ( a ) Immunofluorescence staining of PTEN ( red ) and actin detected by phalloidin antibody ( green ) in human and rat corneas. Cell nuclei were stained with DAPI ( blue ). Scale bar = 20 µm. N = 3. ( b ) Flat-mount staining of PTEN ( red ) in human and rat corneal endothelium. Cell nuclei were stained with DAPI ( blue ). Scale bar = 10 µm. N = 3. ( c, d ) Total protein was extracted from the corneal endothelium detached from human corneoscleral rims and rat corneas. Cell lysates were analyzed by western blotting and immunoblotted using an anti-PTEN antibody. Data are presented as the mean ± SD from three independent experiments and plotted as PTEN protein expression compared with the respective GAPDH control. ** P < 0.01 by the Student's t -test. ( e ) Total protein extracted from human ( N = 9) and rat ( N = 6) corneal endothelium tissue were analyzed by <t>ELISA.</t> Data are presented as the mean ± SD from three independent experiments and plotted as concentration of PTEN in the corneal endothelium (ng/mL). *** P < 0.001 by the Student's t -test.
Sandwich Type Elisa Kit For Rats, supplied by USCN Life, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+ELISA+Kits/10__3390_slash_molecules21111523-160-10-14?v=USCN+Life
Average 90 stars, based on 1 article reviews
sandwich-type elisa kit for rats - by Bioz Stars, 2026-07
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elisa kits for rat cre (1012)  (Exocell Inc)
90
Exocell Inc elisa kits for rat cre (1012)
Differential expressions of <t>PTEN</t> in the human and rat corneal endothelium. ( a ) Immunofluorescence staining of PTEN ( red ) and actin detected by phalloidin antibody ( green ) in human and rat corneas. Cell nuclei were stained with DAPI ( blue ). Scale bar = 20 µm. N = 3. ( b ) Flat-mount staining of PTEN ( red ) in human and rat corneal endothelium. Cell nuclei were stained with DAPI ( blue ). Scale bar = 10 µm. N = 3. ( c, d ) Total protein was extracted from the corneal endothelium detached from human corneoscleral rims and rat corneas. Cell lysates were analyzed by western blotting and immunoblotted using an anti-PTEN antibody. Data are presented as the mean ± SD from three independent experiments and plotted as PTEN protein expression compared with the respective GAPDH control. ** P < 0.01 by the Student's t -test. ( e ) Total protein extracted from human ( N = 9) and rat ( N = 6) corneal endothelium tissue were analyzed by <t>ELISA.</t> Data are presented as the mean ± SD from three independent experiments and plotted as concentration of PTEN in the corneal endothelium (ng/mL). *** P < 0.001 by the Student's t -test.
Elisa Kits For Rat Cre (1012), supplied by Exocell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+ELISA+Kits/10__1096_slash_fj__201801865rr-44-0-14?v=Exocell+Inc
Average 90 stars, based on 1 article reviews
elisa kits for rat cre (1012) - by Bioz Stars, 2026-07
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human bdnf elisa kit  (Hangzhou Eastbiopharm Co)
90
Hangzhou Eastbiopharm Co human bdnf elisa kit
Differential expressions of <t>PTEN</t> in the human and rat corneal endothelium. ( a ) Immunofluorescence staining of PTEN ( red ) and actin detected by phalloidin antibody ( green ) in human and rat corneas. Cell nuclei were stained with DAPI ( blue ). Scale bar = 20 µm. N = 3. ( b ) Flat-mount staining of PTEN ( red ) in human and rat corneal endothelium. Cell nuclei were stained with DAPI ( blue ). Scale bar = 10 µm. N = 3. ( c, d ) Total protein was extracted from the corneal endothelium detached from human corneoscleral rims and rat corneas. Cell lysates were analyzed by western blotting and immunoblotted using an anti-PTEN antibody. Data are presented as the mean ± SD from three independent experiments and plotted as PTEN protein expression compared with the respective GAPDH control. ** P < 0.01 by the Student's t -test. ( e ) Total protein extracted from human ( N = 9) and rat ( N = 6) corneal endothelium tissue were analyzed by <t>ELISA.</t> Data are presented as the mean ± SD from three independent experiments and plotted as concentration of PTEN in the corneal endothelium (ng/mL). *** P < 0.001 by the Student's t -test.
Human Bdnf Elisa Kit, supplied by Hangzhou Eastbiopharm Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+ELISA+Kits/pmc05353043-97-7-11?v=Hangzhou+Eastbiopharm+Co
Average 90 stars, based on 1 article reviews
human bdnf elisa kit - by Bioz Stars, 2026-07
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rat nadph oxidase 4 elisa kit  (EIAab Inc)
90
EIAab Inc rat nadph oxidase 4 elisa kit
Differential expressions of <t>PTEN</t> in the human and rat corneal endothelium. ( a ) Immunofluorescence staining of PTEN ( red ) and actin detected by phalloidin antibody ( green ) in human and rat corneas. Cell nuclei were stained with DAPI ( blue ). Scale bar = 20 µm. N = 3. ( b ) Flat-mount staining of PTEN ( red ) in human and rat corneal endothelium. Cell nuclei were stained with DAPI ( blue ). Scale bar = 10 µm. N = 3. ( c, d ) Total protein was extracted from the corneal endothelium detached from human corneoscleral rims and rat corneas. Cell lysates were analyzed by western blotting and immunoblotted using an anti-PTEN antibody. Data are presented as the mean ± SD from three independent experiments and plotted as PTEN protein expression compared with the respective GAPDH control. ** P < 0.01 by the Student's t -test. ( e ) Total protein extracted from human ( N = 9) and rat ( N = 6) corneal endothelium tissue were analyzed by <t>ELISA.</t> Data are presented as the mean ± SD from three independent experiments and plotted as concentration of PTEN in the corneal endothelium (ng/mL). *** P < 0.001 by the Student's t -test.
Rat Nadph Oxidase 4 Elisa Kit, supplied by EIAab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+ELISA+Kits/pmc07175316-40-9-15?v=EIAab+Inc
Average 90 stars, based on 1 article reviews
rat nadph oxidase 4 elisa kit - by Bioz Stars, 2026-07
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the human and rat calponin 2 elisa kits  (MyBiosource Biotechnology)
90
MyBiosource Biotechnology the human and rat calponin 2 elisa kits
CNN2 modifies lipid accumulation and fatty acid oxidation pathway amid kidney fibrosis. (A, B) The enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> showed triglyceride contents in serum (A) and kidney (B) collected from ShNC and ShCNN2 mice after IRI. Graphs are presented as means ± SEM. ∗P < 0.05 (n = 6). (C) Representative micrographs of Oil Red-O staining in the fibrotic kidneys from ShNC and ShCNN2 mice after IRI. Arrows indicate lipid accumulation in tubules. Scale bar, 50 μm. (D) Western blot assay demonstrated perilipin 2 (Plin2) protein expression in ShNC and ShCNN2 mice fibrotic kidneys after IRI. Numbers indicate individual animals within each group. (E) Immunohistochemical staining showed Plin2 distributions in the fibrotic kidneys of ShNC and ShCNN2 mice after IRI. Arrows indicate positive staining. Scale bar, 50 μm. (F) ELISA showed ATP levels in the total kidneys collected from ShNC and ShCNN2 mice after IRI. Graphs are presented as means ± SEM. ∗P < 0.05 (n = 6). (G) Quantitative RT-PCR analyses revealed the mRNA abundance of PPARα, CPT1α, ACOX1-3, Acsm1, Acsm2, Acsm5, Acadm, and Acadl in fibrotic kidneys from ShNC and ShCNN2 mice after IRI. Graphs are presented as means ± SEM. ∗P < 0.05 (n = 6). (H) Western blot assays demonstrated PPARα, PGC1α, CPT1α, ACOX1, Acsm5, and Acadm protein expression in ShNC and ShCNN2 mice fibrotic kidneys after IRI. Numbers indicate individual animals within each group. (I) Representative micrographs of PPARα, CPT1α, ACOX1, and Acadm staining in the fibrotic kidneys from ShNC and ShCNN2 mice after IRI. Boxed areas are enlarged. Arrows indicate positive staining. Scale bar, 50 μm.
The Human And Rat Calponin 2 Elisa Kits, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+ELISA+Kits/pmc10090436-121-1-15?v=MyBiosource+Biotechnology
Average 90 stars, based on 1 article reviews
the human and rat calponin 2 elisa kits - by Bioz Stars, 2026-07
90/100 stars
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Image Search Results


Differential expressions of PTEN in the human and rat corneal endothelium. ( a ) Immunofluorescence staining of PTEN ( red ) and actin detected by phalloidin antibody ( green ) in human and rat corneas. Cell nuclei were stained with DAPI ( blue ). Scale bar = 20 µm. N = 3. ( b ) Flat-mount staining of PTEN ( red ) in human and rat corneal endothelium. Cell nuclei were stained with DAPI ( blue ). Scale bar = 10 µm. N = 3. ( c, d ) Total protein was extracted from the corneal endothelium detached from human corneoscleral rims and rat corneas. Cell lysates were analyzed by western blotting and immunoblotted using an anti-PTEN antibody. Data are presented as the mean ± SD from three independent experiments and plotted as PTEN protein expression compared with the respective GAPDH control. ** P < 0.01 by the Student's t -test. ( e ) Total protein extracted from human ( N = 9) and rat ( N = 6) corneal endothelium tissue were analyzed by ELISA. Data are presented as the mean ± SD from three independent experiments and plotted as concentration of PTEN in the corneal endothelium (ng/mL). *** P < 0.001 by the Student's t -test.

Journal: Investigative Ophthalmology & Visual Science

Article Title: PTEN Inhibition Accelerates Corneal Endothelial Wound Healing through Increased Endothelial Cell Division and Migration

doi: 10.1167/iovs.61.8.19

Figure Lengend Snippet: Differential expressions of PTEN in the human and rat corneal endothelium. ( a ) Immunofluorescence staining of PTEN ( red ) and actin detected by phalloidin antibody ( green ) in human and rat corneas. Cell nuclei were stained with DAPI ( blue ). Scale bar = 20 µm. N = 3. ( b ) Flat-mount staining of PTEN ( red ) in human and rat corneal endothelium. Cell nuclei were stained with DAPI ( blue ). Scale bar = 10 µm. N = 3. ( c, d ) Total protein was extracted from the corneal endothelium detached from human corneoscleral rims and rat corneas. Cell lysates were analyzed by western blotting and immunoblotted using an anti-PTEN antibody. Data are presented as the mean ± SD from three independent experiments and plotted as PTEN protein expression compared with the respective GAPDH control. ** P < 0.01 by the Student's t -test. ( e ) Total protein extracted from human ( N = 9) and rat ( N = 6) corneal endothelium tissue were analyzed by ELISA. Data are presented as the mean ± SD from three independent experiments and plotted as concentration of PTEN in the corneal endothelium (ng/mL). *** P < 0.001 by the Student's t -test.

Article Snippet: The quantity of PTEN in human and rat corneal endothelium tissues was measured using Human and Rat PTEN ELISA Kits (ELK Biotechnology, Wuhan, China), respectively, according to the manufacturer protocol.

Techniques: Immunofluorescence, Staining, Western Blot, Expressing, Control, Enzyme-linked Immunosorbent Assay, Concentration Assay

Inhibition of PTEN with bpV(pic) contributed to alleviate the TGF-β2-induced G1-arrest. Cell cycle synchronization of B4G12 cells was achieved by starvation in DMEM without FBS for 24 hours. B4G12 cells were then treated with 10 ng/mL TGF-β2, 10 µM bpV(pic), or the combination of the two supplemented in DMEM with 5% FBS. ( a ) After treatment for another 24 hours, cells were collected and underwent flow cytometry analysis. ( b ) Quantification data are presented as the mean ± SD from three independent experiments. *** P < 0.001 by 1-way ANOVA. ( c ) BrdU immunofluorescence staining ( green ) was used to label S-phase cells after drug treatment ( blue signals show nuclei counterstaining with DAPI). ( d ) The percentage of BrdU-positive cells are presented as the mean ± SD from three independent experiments. Scale bar = 50 µm. * P < 0.05, ** P < 0.01, *** P < 0.001 by 1-way ANOVA.

Journal: Investigative Ophthalmology & Visual Science

Article Title: PTEN Inhibition Accelerates Corneal Endothelial Wound Healing through Increased Endothelial Cell Division and Migration

doi: 10.1167/iovs.61.8.19

Figure Lengend Snippet: Inhibition of PTEN with bpV(pic) contributed to alleviate the TGF-β2-induced G1-arrest. Cell cycle synchronization of B4G12 cells was achieved by starvation in DMEM without FBS for 24 hours. B4G12 cells were then treated with 10 ng/mL TGF-β2, 10 µM bpV(pic), or the combination of the two supplemented in DMEM with 5% FBS. ( a ) After treatment for another 24 hours, cells were collected and underwent flow cytometry analysis. ( b ) Quantification data are presented as the mean ± SD from three independent experiments. *** P < 0.001 by 1-way ANOVA. ( c ) BrdU immunofluorescence staining ( green ) was used to label S-phase cells after drug treatment ( blue signals show nuclei counterstaining with DAPI). ( d ) The percentage of BrdU-positive cells are presented as the mean ± SD from three independent experiments. Scale bar = 50 µm. * P < 0.05, ** P < 0.01, *** P < 0.001 by 1-way ANOVA.

Article Snippet: The quantity of PTEN in human and rat corneal endothelium tissues was measured using Human and Rat PTEN ELISA Kits (ELK Biotechnology, Wuhan, China), respectively, according to the manufacturer protocol.

Techniques: Inhibition, Flow Cytometry, Immunofluorescence, Staining

Inhibition of PTEN with bpV(pic) enhanced the promotion effect of TGF-β2 on CEC migration through activating the PI3K/Akt signaling pathway. ( a ) A scratch on the confluent B4G12 monolayer was made, followed by treatment with different drugs. Wound healing was recorded during the next 6 hours. Scale bar = 100 µm. N = 3. ( b ) Cell invasion assay was conducted and after drug treatment for 12 hours, the migrating cells were visualized through crystal violet staining. Scale bar = 100 µm. N = 3.

Journal: Investigative Ophthalmology & Visual Science

Article Title: PTEN Inhibition Accelerates Corneal Endothelial Wound Healing through Increased Endothelial Cell Division and Migration

doi: 10.1167/iovs.61.8.19

Figure Lengend Snippet: Inhibition of PTEN with bpV(pic) enhanced the promotion effect of TGF-β2 on CEC migration through activating the PI3K/Akt signaling pathway. ( a ) A scratch on the confluent B4G12 monolayer was made, followed by treatment with different drugs. Wound healing was recorded during the next 6 hours. Scale bar = 100 µm. N = 3. ( b ) Cell invasion assay was conducted and after drug treatment for 12 hours, the migrating cells were visualized through crystal violet staining. Scale bar = 100 µm. N = 3.

Article Snippet: The quantity of PTEN in human and rat corneal endothelium tissues was measured using Human and Rat PTEN ELISA Kits (ELK Biotechnology, Wuhan, China), respectively, according to the manufacturer protocol.

Techniques: Inhibition, Migration, Invasion Assay, Staining

A model for the mechanism of PTEN regulating corneal endothelial wound healing. PTEN inhibition with bpV(pic) released CECs from TGF-β2-induced G1-arrest and promoted CEC migration in synergy with TGF-β2 through regulating p27Kip1 and PI3K/Akt pathway, thus improved corneal endothelial regeneration in situ in a rat corneal endothelial injury model.

Journal: Investigative Ophthalmology & Visual Science

Article Title: PTEN Inhibition Accelerates Corneal Endothelial Wound Healing through Increased Endothelial Cell Division and Migration

doi: 10.1167/iovs.61.8.19

Figure Lengend Snippet: A model for the mechanism of PTEN regulating corneal endothelial wound healing. PTEN inhibition with bpV(pic) released CECs from TGF-β2-induced G1-arrest and promoted CEC migration in synergy with TGF-β2 through regulating p27Kip1 and PI3K/Akt pathway, thus improved corneal endothelial regeneration in situ in a rat corneal endothelial injury model.

Article Snippet: The quantity of PTEN in human and rat corneal endothelium tissues was measured using Human and Rat PTEN ELISA Kits (ELK Biotechnology, Wuhan, China), respectively, according to the manufacturer protocol.

Techniques: Inhibition, Migration, In Situ

CNN2 modifies lipid accumulation and fatty acid oxidation pathway amid kidney fibrosis. (A, B) The enzyme-linked immunosorbent assay (ELISA) showed triglyceride contents in serum (A) and kidney (B) collected from ShNC and ShCNN2 mice after IRI. Graphs are presented as means ± SEM. ∗P < 0.05 (n = 6). (C) Representative micrographs of Oil Red-O staining in the fibrotic kidneys from ShNC and ShCNN2 mice after IRI. Arrows indicate lipid accumulation in tubules. Scale bar, 50 μm. (D) Western blot assay demonstrated perilipin 2 (Plin2) protein expression in ShNC and ShCNN2 mice fibrotic kidneys after IRI. Numbers indicate individual animals within each group. (E) Immunohistochemical staining showed Plin2 distributions in the fibrotic kidneys of ShNC and ShCNN2 mice after IRI. Arrows indicate positive staining. Scale bar, 50 μm. (F) ELISA showed ATP levels in the total kidneys collected from ShNC and ShCNN2 mice after IRI. Graphs are presented as means ± SEM. ∗P < 0.05 (n = 6). (G) Quantitative RT-PCR analyses revealed the mRNA abundance of PPARα, CPT1α, ACOX1-3, Acsm1, Acsm2, Acsm5, Acadm, and Acadl in fibrotic kidneys from ShNC and ShCNN2 mice after IRI. Graphs are presented as means ± SEM. ∗P < 0.05 (n = 6). (H) Western blot assays demonstrated PPARα, PGC1α, CPT1α, ACOX1, Acsm5, and Acadm protein expression in ShNC and ShCNN2 mice fibrotic kidneys after IRI. Numbers indicate individual animals within each group. (I) Representative micrographs of PPARα, CPT1α, ACOX1, and Acadm staining in the fibrotic kidneys from ShNC and ShCNN2 mice after IRI. Boxed areas are enlarged. Arrows indicate positive staining. Scale bar, 50 μm.

Journal: Molecular Metabolism

Article Title: Calponin 2 harnesses metabolic reprogramming to determine kidney fibrosis

doi: 10.1016/j.molmet.2023.101712

Figure Lengend Snippet: CNN2 modifies lipid accumulation and fatty acid oxidation pathway amid kidney fibrosis. (A, B) The enzyme-linked immunosorbent assay (ELISA) showed triglyceride contents in serum (A) and kidney (B) collected from ShNC and ShCNN2 mice after IRI. Graphs are presented as means ± SEM. ∗P < 0.05 (n = 6). (C) Representative micrographs of Oil Red-O staining in the fibrotic kidneys from ShNC and ShCNN2 mice after IRI. Arrows indicate lipid accumulation in tubules. Scale bar, 50 μm. (D) Western blot assay demonstrated perilipin 2 (Plin2) protein expression in ShNC and ShCNN2 mice fibrotic kidneys after IRI. Numbers indicate individual animals within each group. (E) Immunohistochemical staining showed Plin2 distributions in the fibrotic kidneys of ShNC and ShCNN2 mice after IRI. Arrows indicate positive staining. Scale bar, 50 μm. (F) ELISA showed ATP levels in the total kidneys collected from ShNC and ShCNN2 mice after IRI. Graphs are presented as means ± SEM. ∗P < 0.05 (n = 6). (G) Quantitative RT-PCR analyses revealed the mRNA abundance of PPARα, CPT1α, ACOX1-3, Acsm1, Acsm2, Acsm5, Acadm, and Acadl in fibrotic kidneys from ShNC and ShCNN2 mice after IRI. Graphs are presented as means ± SEM. ∗P < 0.05 (n = 6). (H) Western blot assays demonstrated PPARα, PGC1α, CPT1α, ACOX1, Acsm5, and Acadm protein expression in ShNC and ShCNN2 mice fibrotic kidneys after IRI. Numbers indicate individual animals within each group. (I) Representative micrographs of PPARα, CPT1α, ACOX1, and Acadm staining in the fibrotic kidneys from ShNC and ShCNN2 mice after IRI. Boxed areas are enlarged. Arrows indicate positive staining. Scale bar, 50 μm.

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) The human and rat calponin 2 ELISA kits were purchased from MyBioSource, Inc.

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Western Blot, Expressing, Immunohistochemical staining, Quantitative RT-PCR